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1ac. Pairs of pants, not individually wrapped, can't be dirty (10)
I have S - A - O - R - O - .

Also, 16dn. Gardeners planted around lines in city (6) R - O - E - .
I guess "Rooted" - but why?

1 stainproof
anagram

16 Rhodes ?

Thank you eirlys. Thought of "Rhodes" - but I can't parse for either gardeners or possibly lines = roads???

the RHS and Ode?

Lines : ode
RHS : Royal Horticultural Society

Thank you Trevor & eirlys - I always thought of Rhodes as island or explorer; never the city.

I have just checked. Rhodes is the principal city on the island of Rhodes