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24a. Agitated ants over the girls top? and Ants in ones pants. (5) Letters. A?G?N.
16d. Unwritten rules that make borders more taxing? (7) Letters. C???O?S.
And last one. 21a (6) letters. Emplouing a group of Kathmandu singers. ???N?.
I am not too clever with Anagrams.

Agitated ants over the girls top?- an g st?
"using" is in the last clue

Customs?

16 D. Customs

Dear AB, I don't understand your answer to 24a. did you mean angst? am I wrong with 20d then, Betray? Ruthless and Treacherous old nasties first of all (3,2) Letters. I Have
Run In. Maybe this is wrong. I just hate anagrams, but realy want to master them.

It's rat on. first letter of words.

I think that AB did mean that "Angst" was the answer.
Which letters would that leave you with for the 3,2 one?

Aha! Ignore my post - I see that the answer was en route as I wrote.

Thanks all, I can see the answers now, that I have been told them. I will keep struggling on with the anagrams and cryptic clues.